ORIGINAL RESEARCH ARTICLE

Antimicrobial susceptibility patterns of Acinetobacter baumanii isolates from ICU and non-ICU wards

Ni Nyoman Sri Budayanti , I Wayan Suranadi, Made Adi Tarini, Gusti Ayu Dianti Violentina, I Dewa Gde Sathya Deva

Ni Nyoman Sri Budayanti
Department of Microbiology, Faculty of Medicine; Udayana One Health Collaborating Centre Faculty of Medicine, Udayana University, Sanglah General Hospital, Denpasar-Bali, Indonesia. Email: nyomansribudayanti@gmail.com

I Wayan Suranadi
Department of Anesthesiology, Pain Management, and Intensive Care Faculty of Medicine, Udayana University, Sanglah General Hospital, Denpasar-Bali, Indonesia

Made Adi Tarini
Department of Microbiology, Faculty of Medicine Udayana University, Sanglah General Hospital, Denpasar-Bali, Indonesia

Gusti Ayu Dianti Violentina
Udayana One Health Collaborating Centre Faculty of Medicine, Udayana University, Sanglah General Hospital, Denpasar-Bali, Indonesia

I Dewa Gde Sathya Deva
Udayana One Health Collaborating Centre Faculty of Medicine, Udayana University, Sanglah General Hospital, Denpasar-Bali, Indonesia
Online First: April 30, 2019 | Cite this Article
Budayanti, N., Suranadi, I., Tarini, M., Violentina, G., Sathya Deva, I. 2019. Antimicrobial susceptibility patterns of Acinetobacter baumanii isolates from ICU and non-ICU wards. Bali Journal of Anesthesiology 3(1): 50-54. DOI:10.15562/bjoa.v3i1.147


ABSTRACT

Introduction: Acinetobacter baumanii is the most common agent of hospital-acquired infection with the increasing fatality rate due to multidrug-resistant (MDR) strain infection. The magnitude of the problem in Indonesia is unknown. Here, we provide data regarding susceptibility pattern of A. baumanii isolated from a tertiary referral hospital in Bali, Indonesia between 2012 and 2014.

Methods: Data were collected retrospectively from culture-based records in the Clinical Microbiology department, Sanglah General Hospital during 2012-2014. A. baumanii was isolated from clinical specimens. Identification and antimicrobial susceptibility test were conducted using micro-dilution method (Vitek-2 Compact system). Isolates that resistant to ≥ 3 antibiotic classes were categorized as multi-drug resistant (MDR) A. baumanii.

Results: A. baumanii collected from sputum in intensive care unit (ICU) wards were 7.9%, 11.1%, and 7.0%, while the isolates from sputum in non-ICU wards were 13.1%, 15.6%, and 19.9% in 2012, 2013, and 2014, respectively.  There was a reduced susceptibility of A. baumanii to ciprofloxacin, levofloxacin, ceftazidime, aztreonam, imipenem, ampicillin-sulbactam, and piperacillin-tazobactam in ICU ward. Meanwhile, the susceptibility of A. baumanii to Cotrimoxazole remained high in both ICU and non-ICU ward. MDR A. baumanii is found to be resistant to fluoroquinolones, cephalosporins, aztreonam, aminoglycosides, beta-lactamase inhibitors, and carbapenem. Data were analyzed and presented in a descriptive manner.

Conclusion: Three years surveillance showed that the susceptibility of A. baumanii to most common antibiotics was decreasing. MDR A. baumanii was found to be resistant to all classes of common antibiotics mostly from ICU ward isolates. 

References

REFERENCES

Lin MF, Lan CY. Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside. World J Clin Cases. 2014; 2(12): 787-814. DOI: 10.12998/wjcc.v2.i12.787.

Karuniawati A, Saharman YR, Lestari DC. Detection of carbapenemase encoding genes Acinetobacter baumanii isolated from patients at intensive care unit Cipto Mangunkusumo Hospital in 2011. Acta Med Indones. 2013; 45(2): 101–6. Available at: www.inaactamedica.org/archives/2013/23770789.pdf

Cucunawangsih, Wiwing V, Lugito NPH. Antimicrobial susceptibility of multidrug-resistant Acinetobacter baumanii in a teaching hospital: A two-year observation. Open J Med Microbiol. 2015; 5: 85–9. DOI: 10.4236/ojmm.2015.52010

Cucunawangsih, Wiwing V, Lumbuun N, et al. Increased number of metallo- or OXA carbapenemase producing Acinetobacter baumanii isolated from Tangerang, Indonesia. Arch Clin Microbiol. 2016; 7(3): 1–5. DOI: 10.4172/1989-8436.100045

Garcia LS (ed). Clinical microbiology procedures handbook, 3rd Edition [Internet]. American Society for Microbiology Press; 2010.

CLSI. Performance standards for antimicrobial susceptibility testing. 27th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 27th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute 2017. p:282.

Koo SH, Kwon KC, Cho HH, et al. Genetic basis of multidrug-resistant Acinetobacter baumannii clinical isolates from three university hospitals in Chungcheong Province, Korea. Korean J Lab Med. 2010; 30(5): 498–506. DOI: 10.3343/kjlm.2010.30.5.498.

Tsakiridou E, Makris D, Daniil Z, et al. Acinetobacter baumannii infection in prior ICU bed occupants is an independent risk factor for subsequent cases of ventilator-associated pneumonia. Biomed Res Int. 2014; 2014(Article ID 193516). DOI: 10.1155/2014/193516

Dent LL, Marshall DR, Pratap S, et al. Multidrug-resistant Acinetobacter baumannii: A descriptive study in a city hospital. BMC Infect Dis. 2010; 10:196. DOI: 10.1186/1471-2334-10-196.


No Supplementary Material available for this article.
Article Views      : 58
PDF Downloads : 26